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Plant Analysis Reference Procedures,i ธันวาคม 15, 2010

ผ่านทาง scsb-PlantAnalysis.

The publication can be downloaded from Plant Analysis Reference Procedures for the Southern Region of the United States.

Plant Anal. Ref. Proc. for S. US (SCSB # 368)
PLANT ANALYSIS REFERENCE PROCEDURES
FOR THE SOUTHERN REGION
OF THE UNITED STATES
SOUTHERN COOPERATIVE SERIES
BULLETIN #368
MAY ‘92
URL: http://www.cropsoil.uga.edu/~oplank/sera368.pdf
Dr. C. Owen Plank
The University of Georgia
Crop & Soil Science Dept.
Athens, GA 30602-7272

http://www.cropsoil.uga.edu/~oplank

E-mail: oplank@arches.uga.edu
ISBN: 1-58161-368-7

 

Plant Analysis Reference Procedures,ii

ผ่านทาง scsb-PlantAnalysis.

The publication can be downloaded from Plant Analysis Reference Procedures for the Southern Region of the United States.

Plant Anal. Ref. Proc. for S. US (SCSB # 368)
May 1992 SCSB #368
PLANT ANALYSIS REFERENCE PROCEDURES FOR
THE SOUTHERN REGION OF THE UNITED STATES
Editor
C.O. Plank
ABSTRACT
This bulletin is the instrument used by the Southern Extension Research Activity-Information Exchange Group-6 (SERAIEG-
6) to document in summary form procedures used by state university plant analysis programs. This document,
records detailed analytical methodologies that are used by the various laboratories throughout the Southern Region. Other
procedures are available for many of the analyses presented in this publication. For information related to similar
procedures, each state university maintains laboratory manuals that may be of further assistance to the reader. The intent
of this document is to provide a reference for current and most widely used plant analysis methods.

 

Plant Analysis Reference Procedures,iii

ผ่านทาง scsb-PlantAnalysis.

The publication can be downloaded from Plant Analysis Reference Procedures for the Southern Region of the United States.

NOTE: Commercial companies are mentioned in this publication solely for the purpose of providing
specific information. Mention of a company does not constitute a guarantee or warranty of its products by
the Agricultural Experiment Stations or an endorsement over products of other companies not mentioned.

 

Plant Analysis Reference Procedures,iv

ผ่านทาง scsb-PlantAnalysis.

The publication can be downloaded from Plant Analysis Reference Procedures for the Southern Region of the United States.

Plant Analysis Reference Procedures for the
Southern Region of the United States
Contents
Sample Preparation ………………………………………………………………………………………………………………1
C. R. Campbell and C. O. Plank
A. Decontamination…………………………………………………………………………………………………..1
B. Drying……………………………………………………………………………………………………………….2
C. Particle-Size Reduction …………………………………………………………………………………………..3
D. Storage………………………………………………………………………………………………………………4
E. Organic Matter Destruction-Dry Ashing ………………………………………………………………………5
F. Organic Matter Destruction-Wet Ashing ………………………………………………………………………7
G. Organic Matter Destruction-Accelerated Wet Digestion ……………………………………………………9
Determination of Total Nitrogen in Plant Samples by Kjeldahl ………………………………………………………. 13
W.H. Baker and T. L. Thompson
Determination of Nitrogen in Plant Tissue Using Continuous Flow, Segmented
Stream AutoAn alyzer …………………………………………………………………………………………………………. 17
R. A. Isaac and W. C. Johnson, Jr.
Determination of Total Nitrogen in Plant Tissue by Combustion…………………………………………………….. 20
C. R. Campbell
Determination of Nitrate Nitrogen in Plant Samples by Selective Ion Electrode ………………………………….. 23
W. H. Baker and T. L. Thompson
Determination of Phosphorus in Plant Tissue by Colorimetry ………………………………………………………… 27
K. P. Moore
Determination of Potassium, Calcium, and Magnesium in Plants by Atomic Absorption
Techniques …………………………………………………………………………………………………………………….. 30
E. A. Hanlon
Determination of P, K, Ca, Mg, Mn, Fe, Al, B, Cu, and Zn in Plant Tissue by Inductively
Coupled Plasma (ICP) Emission Spectroscopy ………………………………………………………………………….. 34
S. J. Donohue and D. W. Aho
Determination of P, K, Ca, Mg, Mn, Fe, Al, B, Cu, and Zn in Plant Tissue by Emission
Spectroscopy…………………………………………………………………………………………………………………… 38
R. A. Isaac and W. C. Johnson, Jr.
Determination of Sulfur in Plant Tissue by Combustion……………………………………………………………….. 42
R. A. Isaac and W. C. Johnson, Jr.
Determination of Sulfur in Plant Tissue by Turbidimetry ……………………………………………………………… 45
C. C. Mitchell

 

Plant Analysis Reference Procedures,v

ผ่านทาง scsb-PlantAnalysis.

The publication can be downloaded from Plant Analysis Reference Procedures for the Southern Region of the United States.

Determination of Total Manganese, Iron, Copper and Zinc in Plants by Atomic Absorption
Techniques …………………………………………………………………………………………………………………….. 48
E. A. Hanlon
Determination of Boron in Plants by Azomethine-H Method …………………………………………………………. 51
W. E. Sabbe
Determination of Total Boron in Plants by Curcumin Method………………………………………………………… 54
J. W. Odom
Determination of Molybdenum in Plants………………………………………………………………………………….. 57
J. L. Sims and J. D. Crutchfield
Determination of Molybdenum in Plant Tissue Using the Graphite Furnace ………………………………………. 64
D. O. Wilson
Conversion Table ………………………………………………………………………………………………………………. 68

 

Plant Analysis Reference Procedures,vi

ผ่านทาง scsb-PlantAnalysis.

The publication can be downloaded from Plant Analysis Reference Procedures for the Southern Region of the United States.

This bulletin is one in a series of the Southern Cooperative Series and, as such, is in effect a separate publication by each
of the cooperating agencies listed below. Thus, it may be mailed under the frank and indicia of each. Requests for copies
from outside the cooperating states may be addressed to the Georgia Agricultural Experiment Station, Room 2375,
Coliseum, University of Georgia, Athens, Georgia 30602.
Stations and agencies directly participating are as follows:
AGRICULTURAL EXPERIMENT STATIONS
Alabama Agricultural Experiment Station
Auburn University
Auburn, AL 36849-5403
L. T. Frobish, Director
Arkansas Agricultural Experiment Station
University of Arkansas
Fayetteville, AR 72701
G. J. Musick, Director
Florida Institute of Food and Agricultural Sciences
University of Florida
Gainesville, FL 32611
J. M. Davidson, Director
Georgia Agricultural Experiment Station
University of Georgia
Athens, GA 30602
C. W. Donoho, Jr.
Kentucky Agricultural Experiment Station
University of Kentucky
Lexington, KY 40546-0091
C. O. Little, Director
Louisiana Agricultural Experiment Station
Louisiana State University and A&M College
Baton Rouge, LA 70894
K. W. Tipton, Director
Mississippi Agricultural and Forestry
Experiment Station
Mississippi State University
Mississippi State, MS 39762
V. G. Hurt, Director
North Carolina Agricultural Research Service
North Carolina State University
Raleigh, NC 27695-7643
R. J. Kuhr, Director
Oklahoma Agricultural Experiment Station
Oklahoma State University
Stillwater, OK 74078-0500
C. B. Browning, Director
Puerto Rico Agricultural Experiment Station
University of Puerto Rico
Mayaguez, PR 00708
J. A. Quinones, Acting Director
South Carolina Agricultural Experiment Station
Clemson University
Clemson, SC 29634-0351
J. R. Fischer, Director
Tennessee Agricultural Experiment Station
University of Tennessee
Knoxville, TN 37901
D. O. Richardson, Director
Texas Agricultural Experiment Station
Texas A&M University System
University Station
College, TX 77843-2147
R. G. Merrifield, Director
Virginia Agricultural Experiment Station
Virginia Polytechnic Institute and State University
Blacksburg, VA 24061-0402
J. R. Nichols, Director
Virgin Islands Agricultural Experiment Station
College of the Virgin Islands
St. Croix, USVI 00850
D. S. Padda, Director